Quick print tlc3/1/2023 High precision methods, based chiefly on gas chromatography (GC) or high performance liquid chromatography (HPLC) and capillary electrophoresis have been proposed 5. Many analytical methods have been proposed for detection and evaluation of the quantity of caffeine in distinct materials, such as biological fluids (urine, blood), food and beverages. It is a drug familiar to people of all continents, who consume it every day and are curious about its real effects. It is expected that caffeine consumption will increase even further, due to the widening spread of the fitness culture, the assumption of caffeine thermogenic properties and the increasing popularity of energy drinks.Īnalysis of caffeine is a convenient theme in chemistry education. ![]() Denmark, Finland and Brazil are countries with highest consumption by adult people, daily averages corresponding to 390, 329 and 300 mg, respectively 3. However, the FDA estimation for daily caffeine consumption in 2012 was 300 mg by adults and 100 mg by teen agers 2. Frary and collaborators 1 estimated as 193 mg the average daily consumption per capita in the USA in the period 1994-1998. Care is also necessary regarding pregnant women, since high caffeine intake may have negative consequences on baby delivery and induce low weight of newborns 4.Ĭaffeine consumption has increased over the last decades. Excessive consumption may cause accelerated heart rate, nervousness, anxiety and insomnia, particularly in people still lacking tolerance to caffeine, such as children and teen agers 2. It is admitted that daily doses of caffeine below 250 mg are safe 4. It is commonly assumed that caffeine reduces fatigue and enhances physical endurance, mental alertness and concentration. Food and Drug Administration (FDA) regards caffeine either as a drug or a functional food 3. Common medicines widely consumed possess caffeine as one of their active components 2. ![]() ![]() Worldwide, caffeine is the most consumed alkaloid, mainly in beverages such as tea, coffee and soft drinks. Caffeine (1,3,7-trimethylxanthine) is a stimulant of the central nervous system, relatively abundant in coffee and cacao beans, kola nuts, guarana berries and leaves of tea and yerba mate 1.
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